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dna tethers  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna tethers
    Dna Tethers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tether/Deoxyribonucleic+acid/pmc12664379-68-1-25
    Average 99 stars, based on 1 article reviews
    dna tethers - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Construct:

    Article Title: Cooperative control of a DNA origami force sensor.
    Article Snippet: .. The ND + tether construct is then incubated with streptavidin coated superparamagnetic particles (prerinsed in experiment buffer) (Thermo-Scientific: Dynabeads M-280) for 15 min at room temperature. .. Pre-rinsed 4.5 μm streptavidin labeled polystyrene particles (Spherotech SVP-40–5) are combined with 1 μM biotinylated oligoes that are a reverse complement to the oligo covalently attached to the slide and incubated for 20 min at room temperature.

    Article Title: Soluble lipidated ligand agents for treating eye inflammation
    Article Snippet: .. The nucleotide sequences of all receptor and tether constructs were confirmed using automated DNA sequencing followed by analysis with VectorNTI (Invitrogen). ..

    Article Title: Cooperative control of a DNA origami force sensor
    Article Snippet: .. The ND + tether construct is then incubated with streptavidin coated superparamagnetic particles (prerinsed in experiment buffer) (Thermo-Scientific: Dynabeads M-280) for 15 min at room temperature. .. Pre-rinsed 4.5 μm streptavidin labeled polystyrene particles (Spherotech SVP-40–5) are combined with 1 μM biotinylated oligoes that are a reverse complement to the oligo covalently attached to the slide and incubated for 20 min at room temperature.

    Incubation:

    Article Title: Cooperative control of a DNA origami force sensor.
    Article Snippet: .. The ND + tether construct is then incubated with streptavidin coated superparamagnetic particles (prerinsed in experiment buffer) (Thermo-Scientific: Dynabeads M-280) for 15 min at room temperature. .. Pre-rinsed 4.5 μm streptavidin labeled polystyrene particles (Spherotech SVP-40–5) are combined with 1 μM biotinylated oligoes that are a reverse complement to the oligo covalently attached to the slide and incubated for 20 min at room temperature.

    Article Title: Cooperative control of a DNA origami force sensor
    Article Snippet: .. The ND + tether construct is then incubated with streptavidin coated superparamagnetic particles (prerinsed in experiment buffer) (Thermo-Scientific: Dynabeads M-280) for 15 min at room temperature. .. Pre-rinsed 4.5 μm streptavidin labeled polystyrene particles (Spherotech SVP-40–5) are combined with 1 μM biotinylated oligoes that are a reverse complement to the oligo covalently attached to the slide and incubated for 20 min at room temperature.

    DNA Sequencing:

    Article Title: Soluble lipidated ligand agents for treating eye inflammation
    Article Snippet: .. The nucleotide sequences of all receptor and tether constructs were confirmed using automated DNA sequencing followed by analysis with VectorNTI (Invitrogen). ..

    Imaging:

    Article Title: Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosis
    Article Snippet: .. The tether DNA molecules on the surface were visualized using an imaging buffer containing the T50 buffer along with DNA-Intercalating dye, either 50 nM of Sytox Orange (Thermo Scientific S11368) or 50 nM of YOYO-1 (Thermo Scientific Y3601) and an oxygen scavenging system (1× PCD, 1×PCA and 1×trolox). .. The tether DNA molecules on the surface were visualized using an imaging buffer containing the T50 buffer along with DNA-Intercalating dye, either 50 nM of Sytox Orange (Thermo Scientific S11368) or 50 nM of YOYO-1 (Thermo Scientific Y3601) and an oxygen scavenging system (1× PCD, 1×PCA and 1×trolox).



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    SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa <t>(PEG_0.8k),</t> 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa <t>(20-bp</t> <t>dsDNA),</t> 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.
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    SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa <t>(PEG_0.8k),</t> 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa <t>(20-bp</t> <t>dsDNA),</t> 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.
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    Image Search Results


    SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa (PEG_0.8k), 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa (20-bp dsDNA), 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.

    Journal: MethodsX

    Article Title: In vitro motility-based tether-scanning of the kinesin motor domain

    doi: 10.1016/j.mex.2025.103719

    Figure Lengend Snippet: SDS-PAGE of kinesin-1 monomers tethered at loop 2 . Untethered monomeric kinesin-1 constructs appear as bands around 38 kDa. Calculated molecular weights for tethered constructs were as follows: 39 kDa (PEG_0.8k), 41 kDa (PEG_2k), 44 kDa (PEG_5k), 45 kDa (20-nt ssDNA), 52 kDa (40-nt ssDNA), 57 kDa (60-nt ssDNA), 45 kDa (20-bp dsDNA), 51 kDa (40-bp dsDNA), and 57 kDa (60-bp dsDNA). Labelling efficiencies were 61–100 % for PEG, 7–45 % for ssDNA, and 22–49 % for dsDNA.

    Article Snippet: The specific DNA sequences used were as follows: 20-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCT-biotin-3′ 40-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT-biotin-3′ 60-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT-biotin-3′ 20-bp dsDNA: 5′-amine-TACTAACTAATCGTCCAACG-3′ 5′-biotin-CGTTGGACGATTAGTTAGTA-3′ 40-bp dsDNA: 5′-amine-CGTTGGACACGACAGGTCCTTAGACTGAAATTAGTTAGTA-3′ 5′-biotin-TACTAACTAATTTCAGTCTAAGGACCTGTCGTGTCCAACG-3′ 60-bp dsDNA: 5′-amine-ACTGCCCGCTTTCCAGTCGGGAAACCTGTCGAGCCATCTGCATGAATGAATCGGCCAACG-3′ 5′-biotin-CGTTGGCCGATTCATTCATGCAGATGGCTCGACAGGTTTCCCGACTGGAAAGCGGGCAGT-3′ As PEG tethers, Biotin-PEG11-amine (0.8 kDa, Tokyo Chemical Industry, Tokyo, Japan), Biotin-PEG2k-Maleimide (2 kDa, Biopharma PEG Scientific), and Biotin-PEG5k-Maleimide (5 kDa, Laysan Bio) were purchased.

    Techniques: SDS Page, Construct

    In vitro microtubule gliding assay . (a) Scheme of the in vitro microtubule gliding assay. Single-headed kinesins are tethered to the streptavidin-coated glass surface via tethers using biotin-avidin conjugation. (b) Image of fluorescent microtubules bound to surface-tethered kinesin motor domains via 40-bp DNA linkers. (c) Kymographs of gliding-microtubules driven by kinesin motor domains tethered at loop-2 via PEG_5k, 20-nt ssDNA, and 20-bp dsDNA linkers.

    Journal: MethodsX

    Article Title: In vitro motility-based tether-scanning of the kinesin motor domain

    doi: 10.1016/j.mex.2025.103719

    Figure Lengend Snippet: In vitro microtubule gliding assay . (a) Scheme of the in vitro microtubule gliding assay. Single-headed kinesins are tethered to the streptavidin-coated glass surface via tethers using biotin-avidin conjugation. (b) Image of fluorescent microtubules bound to surface-tethered kinesin motor domains via 40-bp DNA linkers. (c) Kymographs of gliding-microtubules driven by kinesin motor domains tethered at loop-2 via PEG_5k, 20-nt ssDNA, and 20-bp dsDNA linkers.

    Article Snippet: The specific DNA sequences used were as follows: 20-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCT-biotin-3′ 40-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT-biotin-3′ 60-nt ssDNA: 5′-amine-CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT-biotin-3′ 20-bp dsDNA: 5′-amine-TACTAACTAATCGTCCAACG-3′ 5′-biotin-CGTTGGACGATTAGTTAGTA-3′ 40-bp dsDNA: 5′-amine-CGTTGGACACGACAGGTCCTTAGACTGAAATTAGTTAGTA-3′ 5′-biotin-TACTAACTAATTTCAGTCTAAGGACCTGTCGTGTCCAACG-3′ 60-bp dsDNA: 5′-amine-ACTGCCCGCTTTCCAGTCGGGAAACCTGTCGAGCCATCTGCATGAATGAATCGGCCAACG-3′ 5′-biotin-CGTTGGCCGATTCATTCATGCAGATGGCTCGACAGGTTTCCCGACTGGAAAGCGGGCAGT-3′ As PEG tethers, Biotin-PEG11-amine (0.8 kDa, Tokyo Chemical Industry, Tokyo, Japan), Biotin-PEG2k-Maleimide (2 kDa, Biopharma PEG Scientific), and Biotin-PEG5k-Maleimide (5 kDa, Laysan Bio) were purchased.

    Techniques: In Vitro, Gliding Assay, Avidin-Biotin Assay, Conjugation Assay